FastQCFastQC Report
Thu 2 Jun 2016
DRR028521_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028521_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779058
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16350.053.513531
TCAACGC24200.047.9051174
GTATCAA26250.047.774841
ATCAACG24800.046.746123
CAACGCA29050.040.406865
GTACATG50650.038.867311
AACGCAG31150.037.447686
TACATGG49400.037.3416672
TATCAAC31250.037.3310432
ACGCAGA31300.036.3313267
CGCAGAG32700.034.1061178
ACATGGG54300.032.360663
CATGGGG37100.026.1383344
GGACCGT2301.0514705E-625.3585936
GCAGAGT40450.024.3271649
ATACGGG5800.023.8795767
CATGGGA22750.023.0755064
TCTCGGG30550.023.047224145
GTACGGA2852.635461E-723.0229326
GAGTACA40200.022.6717011
Produced by FastQC (version 0.11.5)