FastQCFastQC Report
Thu 2 Jun 2016
DRR028521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779058
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22250.069.303711
GTATCAA38700.055.820811
TCAACGC44300.048.3953824
ATCAACG45250.047.2192763
CAACGCA47600.044.735915
AACGCAG50350.042.148696
TATCAAC51750.041.5682752
ACGCAGA57500.036.0258377
CGCAGAG57700.035.7754368
GTACATG51300.035.0211371
TACATGG52550.032.3900072
ACATGGG52300.030.1905273
GTGGTAT13650.029.8404941
GCAGAGT66650.029.0152979
TGGTATC14050.027.8376982
GTACGGA3201.2569217E-924.8976656
CATGGGG35350.024.382234
AACCGCG1500.001503213824.143197
CAGAGTA63550.022.2246519
GAGTACA40200.021.8932361
Produced by FastQC (version 0.11.5)