FastQCFastQC Report
Thu 2 Jun 2016
DRR028520_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028520_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473589
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14850.047.5420074
ATCAACG15250.046.7722743
GGTATCA11950.043.2436871
CAACGCA17000.041.527925
AACGCAG18000.039.2208146
GTATCAA19950.038.6718751
ACGCAGA20500.033.7265747
GTACATG26650.033.5923351
TATCAAC22400.033.1424372
CGCAGAG21050.031.8059548
GAGCGAC6250.029.967505145
TACATGG28550.028.2975672
TCTCGGG19150.026.332163145
ACATGGG31800.022.2012233
GCAGAGT26450.021.1847279
GGACCGT2154.927509E-420.3109466
GAGTACA24750.020.2911261
AGTACAT23050.019.577312
GTATAGG6450.019.1832181
AGACCGT2358.9668314E-418.5823546
Produced by FastQC (version 0.11.5)