FastQCFastQC Report
Thu 2 Jun 2016
DRR028520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473589
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17590.1193684263386874No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16990.11529673470689589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21000.078.934831
GTATCAA35850.055.363891
TCAACGC39450.049.020594
ATCAACG39600.049.0178153
CAACGCA41350.046.7681355
AACGCAG43900.044.2165226
TATCAAC44900.043.2317432
ACGCAGA48850.039.43957
CGCAGAG49100.038.9436578
GCAGAGT58100.030.7918039
GTACGGA1902.0955667E-730.4965486
GTACATG28000.029.8602161
GTGGTAT10600.028.1210231
CTGTCGC5300.025.9652219
TACATGG29750.025.5632822
AGTACGG2559.582618E-825.5632825
TGGTATC11300.024.3567562
ACATGGG30100.023.3410053
CAGAGTA54900.023.2195959
AGACCCG2653.8140606E-621.865455
Produced by FastQC (version 0.11.5)