FastQCFastQC Report
Thu 2 Jun 2016
DRR028519_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028519_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980343
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15600.047.6810531
TCAACGC20700.045.7976574
ATCAACG21050.045.036173
GTATCAA24850.039.616641
CAACGCA25350.037.3949855
GTACATG44150.035.842581
AACGCAG27050.035.043946
TATCAAC29700.032.4106522
TACATGG46650.032.3585432
ACGCAGA29750.031.3724697
CGCAGAG30300.030.3193728
ACATGGG50400.028.64873
CATGGGA23000.024.0966134
GCAGAGT39950.020.8093413
AGAGTAC33600.020.6173255
CTAGCGT1800.004165516220.2566573
GAGCGAC10400.020.028442145
GAGTACA39400.019.8042241
TCTCGGG32800.019.708393145
CAGAGTA37050.019.682584
Produced by FastQC (version 0.11.5)