FastQCFastQC Report
Thu 2 Jun 2016
DRR028519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980343
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21150.071.133081
GTATCAA42950.060.918751
ATCAACG43750.057.9409683
TCAACGC44000.057.611764
CAACGCA47050.053.8770985
AACGCAG50050.050.6476976
TATCAAC51700.049.591642
ACGCAGA58050.043.293537
CGCAGAG58300.042.6109628
GCAGAGT67500.036.052169
GTACATG45200.036.0181351
TACATGG49400.033.134262
ACATGGG52550.027.8403323
TTGTACG1801.5315627E-424.142079
GAGTACA42550.022.8884751
CATGGGA22550.021.8402774
ATACCGC3655.7607394E-921.8270787
AGTACAT40150.021.6466872
CATGGGG35450.020.6348294
GTGGTAT11350.020.4911231
Produced by FastQC (version 0.11.5)