FastQCFastQC Report
Thu 2 Jun 2016
DRR028518_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028518_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995934
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16400.051.1387671
ATCAACG24500.047.3289953
TCAACGC24800.047.0505334
GTATCAA26700.046.4337651
GTACATG52300.040.1593861
TACATGG54950.037.0282142
TATCAAC33300.035.2596442
CAACGCA33350.034.9881065
AACGCAG35550.032.61696
ACGCAGA36300.031.3395047
CGCAGAG37000.030.5479478
ACATGGG63650.030.3629343
CATGGGG43400.025.5417184
GCAGAGT43750.023.0007539
GAGCGAC12700.022.61845145
GAGTACA47300.021.8939171
AGTACAT43650.021.8868522
GTACTAG4006.4210326E-1021.8784981
CATGGGA26000.021.317514
AGAGTAC35950.021.0974865
Produced by FastQC (version 0.11.5)