FastQCFastQC Report
Thu 2 Jun 2016
DRR028518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995934
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24100.070.957531
GTATCAA42000.059.081671
ATCAACG45700.052.617513
TCAACGC46250.052.1483884
CAACGCA50100.048.140985
TATCAAC54050.045.024822
AACGCAG54000.044.7982566
ACGCAGA61550.038.9500777
GTACATG50800.038.676551
CGCAGAG62600.038.181068
TACATGG53250.034.5479662
GCAGAGT72450.031.9904239
ACATGGG58450.030.3591753
GTGGTAT12850.026.0496791
CATGGGG42850.024.6780244
GAGTACA44700.023.7680191
AGTACAT42100.023.2252332
GTACGGA3106.3101106E-721.0275756
CATGGGA23050.020.424474
TGGACCG2557.0150716E-519.8822845
Produced by FastQC (version 0.11.5)