FastQCFastQC Report
Thu 2 Jun 2016
DRR028517_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028517_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972534
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC25150.048.085024
ATCAACG25450.047.804453
GGTATCA19250.045.7924961
GTATCAA28900.044.114351
GTACATG53500.042.6216471
CAACGCA29900.040.6876565
TACATGG54050.039.7617952
AACGCAG33150.036.9184236
ACATGGG58150.034.2020873
ACGCAGA35150.033.9879347
TATCAAC38450.033.3469432
CGCAGAG36300.031.7038568
GAGCGAC10950.027.592377145
CATGGGG40550.025.331914
GCAGAGT43250.023.745019
ATACCGC3402.3737812E-923.567857
TCTCGGG34800.023.358938145
AGTACAT42450.023.1683852
GAGTACA45600.022.3667491
GTATACG2953.7016434E-722.2259431
Produced by FastQC (version 0.11.5)