FastQCFastQC Report
Thu 2 Jun 2016
DRR028517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972534
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24700.072.7593151
GTATCAA41200.054.569481
TCAACGC45250.048.4970634
ATCAACG46300.047.3972433
CAACGCA48100.045.623545
AACGCAG52250.041.999856
TATCAAC55300.040.4692152
GTACATG52650.040.214551
TACATGG53300.037.639512
ACGCAGA59300.036.7623447
CGCAGAG60750.035.5272378
ACATGGG55350.034.0210043
GTGGTAT12650.031.059311
GCAGAGT69450.029.0953379
CATGGGG39150.026.8242554
GAGTACA46400.025.7166561
AGTACAT43800.023.811122
GTCGTAC1851.7877502E-423.5976141
CATGGGA24750.022.5323734
ATACCGC3604.9130904E-922.130017
Produced by FastQC (version 0.11.5)