FastQCFastQC Report
Thu 2 Jun 2016
DRR028516_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028516_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741406
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28390.16302918446358863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG20950.052.7571753
TCAACGC20900.052.1890684
CAACGCA21100.050.9988255
GGTATCA13750.050.3836061
GTATCAA24950.046.3759961
AACGCAG23850.043.8732646
GTACATG39200.041.737941
TATCAAC32000.036.380492
ACGCAGA26650.035.6712347
TACATGG43450.035.611562
CGCAGAG25500.035.5648848
ACATGGG47400.030.3131163
GCAGAGT33200.024.2576719
GAGTACA30750.022.0499111
CTAGTAC4901.4551915E-1121.0526263
GTATTAG16400.020.6717911
CATGGGG34300.019.7677764
CATGGGA23700.019.280024
AGTACCG2307.12994E-419.2270765
TATCCCG1950.006221985418.8984075
Produced by FastQC (version 0.11.5)