FastQCFastQC Report
Thu 2 Jun 2016
DRR028516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741406
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17900.10279050376534823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21850.069.6615751
GTATCAA37700.057.2928431
TCAACGC40500.050.7708784
ATCAACG41150.050.6726953
CAACGCA40750.050.2817275
AACGCAG44850.045.8466156
TATCAAC49150.042.7194672
CGCAGAG49950.040.2958838
ACGCAGA51600.039.5686077
GTACATG42150.038.6910441
TACATGG44050.035.9956132
ACATGGG47300.032.910063
GCAGAGT60900.031.742819
GACGGCG1701.0412367E-425.5536967
CATGGGG33750.023.5977334
CAGAGTA60000.022.5653329
GAGTACA30500.022.100831
CATGGGA23400.021.6587564
AGTACAT33200.018.9728492
AACTGCG2701.090999E-418.7709247
Produced by FastQC (version 0.11.5)