FastQCFastQC Report
Thu 2 Jun 2016
DRR028515_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028515_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610463
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26690.16572873763631948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16900.051.4504934
ATCAACG17600.048.555963
GTATCAA20000.046.7811551
CAACGCA18500.046.208535
AACGCAG19450.043.940336
GTACATG35650.041.1235661
GGTATCA12400.039.212041
TACATGG38650.038.1221772
ACGCAGA22550.037.574237
CGCAGAG22450.035.866668
TATCAAC25150.035.1512262
ACATGGG44000.030.3056143
CTAGCGT1450.001117329525.403833
CATGGGA20850.024.3858174
GTACGGA1600.001981446823.0236916
GCAGAGT29550.022.3399319
GAGTACA26700.020.9700531
CATGGGG30700.020.8821474
AATACCG3051.1079537E-519.3297395
TGACGCC6051.8189894E-1219.020033145
Produced by FastQC (version 0.11.5)