FastQCFastQC Report
Thu 2 Jun 2016
DRR028515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610463
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16890.10487667211230559No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20150.064.0098341
GTATCAA32750.054.4284021
TCAACGC34500.052.260294
ATCAACG35050.051.2336353
CAACGCA36200.049.8060725
AACGCAG38300.047.453316
TATCAAC42600.041.983522
ACGCAGA44350.040.0003667
CGCAGAG45200.039.087958
GTACATG35650.033.3338971
GCAGAGT56400.030.5554989
TACATGG38150.029.2292522
ACATGGG37950.028.810893
GTTAACG1658.4940904E-526.330484145
GTATTAG13800.023.6284481
CATGGGG27800.023.1812444
ACCGTCC1650.002620617921.942078
CAGAGTA53700.019.1472139
TCTAGCG2000.00797576318.102213
GAGTACA27050.017.947721
Produced by FastQC (version 0.11.5)