FastQCFastQC Report
Thu 2 Jun 2016
DRR028514_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028514_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498058
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8110.16283244120162713No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG7600.15259266993000814No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6290.12629051234996727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGA553.6379788E-12107.225582
GGTATCA3800.093.116951
ACATGGA1400.063.18653
CATGGAA1059.531504E-1056.1831934
ATACATG400.005658644755.2996186
TACATGG19500.054.43762
ACCCGGT552.960333E-453.6238676
GTACATG21250.053.4235731
AATACAT450.00900409249.1704525
GTCTTGA450.00902249749.1450581
CATGGGA13200.047.4843754
AACGCAA951.7048096E-646.582545
TGGGATC1750.046.3463446
GGGATCC803.378032E-546.0830157
GTATCAA10800.045.73221
TCAACGC10750.045.27324
ATCAACG10650.044.299773
ATAGATG2200.043.5693936
ACATGGG23650.042.3915063
CAACGCA11000.041.5713845
Produced by FastQC (version 0.11.5)