FastQCFastQC Report
Thu 2 Jun 2016
DRR028514_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028514_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498058
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20140.40437057531452164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19720.39593782250260007No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG12210.24515217103228942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11320.22728276626417004No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG8040.16142698239964018No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT7720.15500202787627143No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC6720.13492404499074406No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG6430.1291014299539411No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA6240.1252866132056909No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA6010.12066867714201959No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC5860.11765697970919049No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA5600.11243670415895338No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG5580.11203514450124283No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT5160.10360239168932134No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG5140.10320083203161076No Hit
TTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCAT5050.10139381357191331No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG5010.10059069425649221No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA4990.10018913459878168No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9050.0123.266661
TCATGGA406.161936E-790.5035252
GCTCTAG608.3309715E-1084.512381
GTTATCA300.001941622272.4391861
ATAGATG3500.066.196876
GTGGACC554.091051E-665.820753
TAGATGG3550.065.264517
GGAGCGC350.003584447862.0595638
TGGTATC1500.057.9222532
GTATCAA22950.057.4463231
TGTGGAC651.1015605E-555.6944772
GGACCAG651.1015605E-555.6944775
GTGGTAT1750.053.8119661
AATAGAT4600.053.515135
GATGACC2650.051.9114579
TACATGG18800.051.221142
ATCAACG26900.049.2554553
CAACGCA26950.049.164075
GGATGAC2950.049.086668
TCAACGC27400.048.356634
Produced by FastQC (version 0.11.5)