FastQCFastQC Report
Thu 2 Jun 2016
DRR028513_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028513_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349559
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21930.16249752696992129No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16150.047.085694
ATCAACG16400.046.3519633
GGTATCA12100.045.7457431
GTATCAA19300.043.5935551
CAACGCA17500.042.609615
GTACATG34050.038.7981221
AACGCAG18850.038.764296
TACATGG35500.036.7962072
CGCAGAG21150.034.685718
ACATGGG37050.034.262183
ACGCAGA21500.033.6443567
TATCAAC24550.032.1655122
CATGGGG28550.024.5664124
GCAGAGT27250.024.5232079
GTATTAG10400.024.1279471
CATGGGA19650.021.791634
CAGAGTA26300.018.5043989
GAGTACA25000.017.712751
AGTACAT27250.017.3329162
GTATTAA13750.017.1761
Produced by FastQC (version 0.11.5)