FastQCFastQC Report
Thu 2 Jun 2016
DRR028513_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028513_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349559
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15270.1131480728148973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13550.10040316873882506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.061.564551
GTATCAA32150.059.7281381
TCAACGC36150.052.6772964
ATCAACG36350.052.5866553
CAACGCA37300.051.247325
AACGCAG40350.047.553056
TATCAAC42400.045.0831342
ACGCAGA45050.041.788297
CGCAGAG45550.040.8527038
GCAGAGT54400.032.875639
GTACATG36050.032.361951
TACATGG38800.028.9251772
ACATGGG37950.028.619073
GAGTACA28400.022.9635091
CATGGGG26900.022.610134
GTGGTAT10300.022.512661
CAGAGTA50800.021.664869
CGTAATA1800.004340225420.1128462
AGTACAT31850.019.7781582
CCTATAC6350.019.3843483
Produced by FastQC (version 0.11.5)