FastQCFastQC Report
Thu 2 Jun 2016
DRR028512_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028512_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587391
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26290.1656176707566063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20100.054.666494
ATCAACG20300.054.4681933
CAACGCA20900.052.573995
AACGCAG21550.048.922316
GTATCAA23400.048.5108071
GGTATCA14900.043.0394441
GTACATG34650.042.12071
ACGCAGA24000.042.087757
CGCAGAG24500.040.0088968
TATCAAC27650.038.9229242
TACATGG38750.036.1434562
ACATGGG40650.032.6407173
GCAGAGT32750.025.0527869
CATGGGG31850.023.3852984
CATGGGA18900.019.899374
GTATTAG12800.019.5795461
TATTACG2751.09422515E-418.7634682
GAGTACA27150.017.6472571
ATAGTAC8550.017.2429523
ACCGTAT3002.3904596E-416.942048
Produced by FastQC (version 0.11.5)