FastQCFastQC Report
Thu 2 Jun 2016
DRR028512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587391
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18980.11956726477597517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20200.075.334161
GTATCAA36850.063.5168651
TCAACGC41500.054.265224
ATCAACG42350.053.1760753
CAACGCA42600.053.033985
AACGCAG44950.050.2613536
TATCAAC48800.047.1863672
ACGCAGA50300.044.1956637
CGCAGAG51100.042.5118148
GTACATG38350.035.5235631
GCAGAGT62350.033.5637639
TACATGG41900.031.280512
ACATGGG42000.029.8267613
CAGAGTA58600.021.7482519
CATGGGG31900.021.3376434
GTATACG1800.00432047120.1289671
GTATTAG12850.019.7373541
ACGGTCC1850.005083820319.5707538
GTGGTAT11100.018.932111
CATGGGA19600.018.8418434
Produced by FastQC (version 0.11.5)