FastQCFastQC Report
Thu 2 Jun 2016
DRR028511_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028511_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510409
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24010.15896356549782212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG21300.049.471613
GGTATCA14950.049.288281
TCAACGC20850.049.140714
GTATCAA23100.049.1239851
CAACGCA21600.046.7519235
AACGCAG22600.044.3495526
GTACATG37700.037.722531
TATCAAC29250.037.2838442
ACGCAGA25700.036.420357
CGCAGAG26050.035.9474378
TACATGG39300.033.1868132
ATTACGC1503.9381746E-529.4753913
ACATGGG46600.026.5658043
GCAGAGT33300.026.1591119
GAGTACA29300.022.8854051
CATGGGG31450.022.0312884
AGTACAT31700.019.2931732
GTACTAG5309.513315E-1018.0739171
CATGGGA22200.017.5976854
GTATTAG12650.016.8924371
Produced by FastQC (version 0.11.5)