FastQCFastQC Report
Thu 2 Jun 2016
DRR028511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510409
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22750.1506214541888985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20360.13479792559498785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22450.071.964891
GTATCAA37900.059.45021
ATCAACG45800.048.6904873
TCAACGC46300.048.164674
CAACGCA47800.046.653235
AACGCAG50600.044.2147146
TATCAAC52250.042.8184622
ACGCAGA56000.039.4339877
CGCAGAG56600.039.0159578
GTACATG38700.033.697191
TGTACCG1751.0216718E-733.0986945
GCAGAGT65150.032.8954739
TACATGG40950.030.7648092
ACATGGG42050.028.7547343
CGCTATA1050.00776000627.5822432
CAGAGTA65350.025.260869
CATGGGG29650.024.4193554
TATAACG1700.003117535721.2951134
GTGGTAT11150.019.4929921
GTATTAG11500.018.8997271
Produced by FastQC (version 0.11.5)