FastQCFastQC Report
Thu 2 Jun 2016
DRR028510_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028510_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193205
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19610.16434728315754626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12100.054.762061
GTATCAA18800.048.952491
TCAACGC18400.046.8317344
ATCAACG18650.046.580823
CAACGCA18900.044.034075
AACGCAG20000.041.238626
GTACATG33050.038.315951
TATCAAC24450.037.0365222
ACGCAGA22350.033.9401057
TACATGG34100.033.896062
CGCAGAG22650.033.3208358
ACATGGG36650.030.3324093
CATGGGA17600.025.1079244
GCAGAGT28700.023.769239
GAGTACA25750.020.8722021
CATGGGG24900.020.7047924
AGAGTAC23500.019.7444425
AGTACAT27750.019.1017652
CAGAGTA27150.018.7176574
TAATACG2000.00723004218.4124774
Produced by FastQC (version 0.11.5)