FastQCFastQC Report
Thu 2 Jun 2016
DRR028510_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028510_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193205
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22920.19208769658189498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22500.18856776496913774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11950.10015043517249761No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18250.085.377321
GTATCAA35950.067.129321
TCAACGC42650.055.694494
ATCAACG42700.055.6292763
CAACGCA43750.054.2941745
TATCAAC46500.051.5504532
AACGCAG46000.051.1661726
CGCAGAG51300.044.6094788
ACGCAGA52500.044.2795457
GCAGAGT58800.038.426849
GTACATG31850.033.4483571
TACATGG32500.030.3049092
ACATGGG33250.028.3145143
CATGGGA16800.021.9845874
GAGTACA25900.021.5455651
CAGAGTA57850.020.905989
CATGGGG22650.020.782734
AGTACAT27350.019.3296032
CCGTGCA2359.2701474E-418.4901669
AGAGTAC54100.017.79226710-14
Produced by FastQC (version 0.11.5)