FastQCFastQC Report
Thu 2 Jun 2016
DRR028509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5499
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1202.182214948172395No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT991.800327332242226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT721.309328968903437No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT561.0183669758137843No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.7274049827241317No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.6001091107474086No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC320.5819239861793053No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA280.5091834879068922No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA270.4909983633387889No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC270.4909983633387889No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA260.4728132387706856No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA230.41825786506637574No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT230.41825786506637574RNA PCR Primer, Index 27 (95% over 23bp)
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG230.41825786506637574No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG210.3818876159301691No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.36370249136206584No Hit
GGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGAACGATCG170.30914711765775593No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT140.2545917439534461No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA140.2545917439534461No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC130.2364066193853428No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC130.2364066193853428No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT120.2182214948172395RNA PCR Primer, Index 27 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC120.2182214948172395No Hit
GCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG120.2182214948172395No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT110.20003637024913623No Hit
GTATCAACGCAGAGTACATGGGAATAAAGGCGCTGCATTGACGGTGCTGA110.20003637024913623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA110.20003637024913623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG110.20003637024913623No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.20003637024913623No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.20003637024913623No Hit
ACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGA110.20003637024913623No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.18185124568103292No Hit
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG100.18185124568103292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC100.18185124568103292No Hit
GTACATGGGAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGG100.18185124568103292No Hit
ATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTA100.18185124568103292No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC100.18185124568103292No Hit
ACCGACGCTGACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCG100.18185124568103292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG90.16366612111292964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA90.16366612111292964No Hit
GCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTG90.16366612111292964No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA80.14548099654482632No Hit
AAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAA80.14548099654482632No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA80.14548099654482632No Hit
CCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAC80.14548099654482632No Hit
ACGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTG80.14548099654482632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC80.14548099654482632No Hit
GACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATG70.12729587197672304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC70.12729587197672304No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA70.12729587197672304No Hit
GTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATA70.12729587197672304No Hit
CATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGAA70.12729587197672304No Hit
CCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCG70.12729587197672304No Hit
CGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAA70.12729587197672304No Hit
GTATCAACGCAGAGTACATGGGAGCTATACGAAGCATTAGGCGAGCGTGC70.12729587197672304No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT70.12729587197672304No Hit
ATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATC70.12729587197672304No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA70.12729587197672304No Hit
CTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACA60.10911074740861974No Hit
CTGACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATC60.10911074740861974No Hit
ACGCAGAGTACATGGGAATAAAGGCGCTGCATTGACGGTGCTGACCCAAC60.10911074740861974No Hit
GTTCATCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAAT60.10911074740861974No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG60.10911074740861974No Hit
ATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATT60.10911074740861974No Hit
AGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCG60.10911074740861974No Hit
GCGTAATTCAGCGCCTCACGGACCTTCGGGTTATCGAACGGCTTTTGCGT60.10911074740861974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC60.10911074740861974No Hit
GTACATGGGAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTT60.10911074740861974No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC60.10911074740861974No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA456.548362E-11112.577981
GTATCAA950.076.180591
TATCAAC1250.057.897252
TCAACGC1351.8189894E-1253.6085664
AACGCAG1403.6379788E-1251.693976
ATCAACG1403.6379788E-1251.693973
CAACGCA1403.6379788E-1251.693975
ACGCAGA1702.3646862E-1142.5715077
CGCAGAG1702.3646862E-1142.5715078
GCAGAGT1753.274181E-1141.3551759
AGTACAT456.715652E-432.165147
CAGAGTA1700.029.8000554
AGAGTAC1700.029.8000555
GAGTACA500.001362965928.9486266
AGTACTT1350.026.8042837
GAGTACT1350.026.8042836
GTACTTT1402.593879E-925.8469858
TACTTTT1454.254616E-924.955719
ACTTTTT1450.019.96456715-19
CTTTTTT2000.014.47431315-19
Produced by FastQC (version 0.11.5)