FastQCFastQC Report
Thu 2 Jun 2016
DRR028508_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028508_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486649
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24170.16258040734564783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17200.054.427314
ATCAACG17750.053.1432343
CAACGCA18300.051.1557275
GGTATCA12700.048.1576921
GTATCAA21350.046.2485581
AACGCAG20550.044.4694026
ACGCAGA22950.038.2133987
TATCAAC25500.037.8588562
GTACATG35350.037.10411
CGCAGAG22900.036.7725688
TACATGG37450.034.830352
ACATGGG42600.028.7167223
GCAGAGT30800.024.9836949
GAGTACA27200.021.4017581
CATGGGA21750.021.351234
GTACTAG5201.8189894E-1221.2558861
CATGGGG30750.019.8964024
GTATATA14300.018.550591
GTATTAG13250.017.7961241
AGTACAT30800.017.7058752
Produced by FastQC (version 0.11.5)