FastQCFastQC Report
Thu 2 Jun 2016
DRR028508_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028508_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486649
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19360.13022576277251724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17590.11831979169259187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.076.770711
GTATCAA33600.061.0505331
TCAACGC37200.053.136674
ATCAACG37950.052.468123
CAACGCA38400.051.4761475
AACGCAG41250.048.0951466
TATCAAC43600.046.1671332
ACGCAGA46400.042.132817
CGCAGAG46250.041.643248
GTACATG33850.034.9036831
GCAGAGT57150.032.560539
TACATGG37600.030.6184982
ACATGGG39100.027.2216953
ACCGCGT1100.00973442226.329468
GTGGTAT10200.024.161341
CAGAGTA53350.021.9864569
TGGTATC10800.020.1127822
CATGGGA18950.019.4865784
TAGTACG4553.4924597E-919.096094
GTATAGT8850.018.8376561
Produced by FastQC (version 0.11.5)