FastQCFastQC Report
Thu 2 Jun 2016
DRR028507_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028507_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419703
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23050.16235790161745095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13000.052.150541
TCAACGC17150.048.9929734
ATCAACG17450.048.136723
GTATCAA20250.045.4882621
CAACGCA18650.044.263735
AACGCAG20400.041.5429886
GTACATG33000.039.5251581
TATCAAC23450.037.077142
ACGCAGA21750.036.931537
TACATGG35100.035.4769822
CGCAGAG23450.034.3569768
ACATGGG38750.030.6140843
GCAGAGT28800.023.943169
CATGGGG30850.022.2188134
GAGTACA26400.021.4932021
CATGGGA19050.020.118774
AGTACAT28550.019.8724632
GTATATA11950.018.4998281
ATTAACG2000.00721201918.4207423
GTATTAG12000.017.8086551
Produced by FastQC (version 0.11.5)