FastQCFastQC Report
Thu 2 Jun 2016
DRR028507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419703
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22290.15700466928646345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19530.1375639834528771No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20700.077.3509751
GTATCAA40050.062.4108921
ATCAACG42150.058.2311333
TCAACGC42150.058.2311334
CAACGCA44150.055.5932545
AACGCAG45900.053.1582076
TATCAAC48350.050.9138032
ACCGCGT1056.9829184E-848.2682848
ACGCAGA52400.046.0114677
CGCAGAG52500.045.372198
GCAGAGT60300.037.9422349
GTACATG34000.032.3898161
TACATGG36750.029.946042
CCGCGTA1050.00776035527.5818779
ACATGGG37250.026.6285443
CCTATAC4900.023.641613
CATGGGG27350.020.3838654
GAGTACA25700.019.45181
GTATATA12800.019.2447721
CTATACA8800.018.9233634
Produced by FastQC (version 0.11.5)