FastQCFastQC Report
Thu 2 Jun 2016
DRR028506_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028506_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329257
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22140.16655921315441635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19550.057.3528754
GTATCAA20500.057.1828731
GGTATCA11850.054.12821
ATCAACG20900.053.978193
CAACGCA20700.053.8102465
AACGCAG22950.048.2039646
ACGCAGA25500.042.805127
TATCAAC26900.042.759112
CGCAGAG25050.042.0325058
GTACATG33400.035.5387461
TACATGG34350.031.5534532
GCAGAGT31900.029.1369639
ACATGGG39400.025.6387883
GAGTACA27050.021.5319061
GTATTAG11300.021.5307031
GTACGGA1850.00457169619.9329916
CATGGGG28000.018.9684944
CATGGGA19150.018.8749454
GTACTAG3551.9510808E-618.6911851
GTGCGCA2000.0078454818.1536949
Produced by FastQC (version 0.11.5)