FastQCFastQC Report
Thu 2 Jun 2016
DRR028506_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028506_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329257
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18120.1363167543973814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17660.13285617453961124No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20050.084.920571
GTATCAA34850.060.08341
ATCAACG41550.049.4857253
TCAACGC41800.049.1897544
CAACGCA43300.047.485725
AACGCAG46000.044.541136
TATCAAC46950.044.2566832
CGCAGAG52300.038.760468
ACGCAGA53650.037.9200747
GTACATG37150.034.1301881
GCAGAGT60100.032.8867389
TACATGG38950.031.2272972
ACATGGG39800.028.7413143
GTACCGT1606.894924E-527.1496266
CATGGGA19450.023.4505774
CAGAGTA56700.021.3238689
GAGTACA28350.020.1898671
CATGGGG26300.019.8202614
GTGGTAT8450.018.8636381
AGAGTAC53100.018.1043310-14
Produced by FastQC (version 0.11.5)