FastQCFastQC Report
Thu 2 Jun 2016
DRR028505_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028505_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300634
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20930.16092151981264521No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20250.054.8803521
TCAACGC19050.053.720394
ATCAACG19150.053.425093
GGTATCA12100.050.484451
CAACGCA20600.049.678325
GTACATG35100.046.3393671
AACGCAG22900.043.39256
TATCAAC25150.042.7281572
TACATGG36950.040.6363752
CGCAGAG24050.038.6335228
ACGCAGA24700.038.1457867
ACATGGG40450.034.572783
CATGGGA20350.028.943094
GCAGAGT28850.027.1736099
GTACGGA1851.652347E-423.8723956
ATATCGC1550.001656153223.7440456
CATGGGG28850.023.2228164
GAGTACA25400.022.0213971
TACGGGT2505.3087388E-520.6147164
GTATTAG11750.020.0436611
Produced by FastQC (version 0.11.5)