FastQCFastQC Report
Thu 2 Jun 2016
DRR028505_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028505_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300634
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21900.16837942111308793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20210.15538575802262589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19850.079.5783541
GTATCAA38850.060.0570221
ATCAACG41650.055.10733
TCAACGC41900.054.6056984
CAACGCA43300.052.8401575
AACGCAG47500.049.2349856
TATCAAC48100.048.169242
CGCAGAG52850.043.0179378
ACGCAGA53650.042.91637
GTACATG35200.037.8768731
GCAGAGT59250.036.7826589
TACATGG35250.035.9454422
ACATGGG38600.032.4506723
CATGGGG25650.024.2759364
GAGTACA27650.023.0614431
GTGGTAT10100.022.240231
CATGGGA19400.021.6466754
TGGTATC10300.021.0886572
CAGAGTA59600.020.7737459
AGTACAT29150.020.6160012
Produced by FastQC (version 0.11.5)