FastQCFastQC Report
Thu 2 Jun 2016
DRR028504_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028504_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2679306
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43430.16209421394943316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC27850.051.6186184
ATCAACG29250.049.132883
CAACGCA29550.047.4025235
AACGCAG31700.043.9439776
GTATCAA35700.043.7671051
GGTATCA23100.039.563061
GTACATG56900.039.1178361
ACGCAGA35950.037.3152247
CGCAGAG34800.036.9092528
TACATGG58950.036.255122
ACATGGG56700.035.3548773
TATCAAC44650.034.3318332
CGATACC16550.032.147247145
CATGGGA34150.025.473484
GCAGAGT45950.023.8469419
CATGGGG38250.022.1647784
TACCGTC2754.5203833E-621.4422747
GTATTAG21700.020.0388341
GAGTACA39550.018.8215351
ATACCGT5701.2914825E-1018.1029786
Produced by FastQC (version 0.11.5)