FastQCFastQC Report
Thu 2 Jun 2016
DRR028504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2679306
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29800.055.9187051
TCAACGC44900.050.9596984
GTATCAA48400.048.949461
CAACGCA46850.048.8386425
ATCAACG47650.048.018693
AACGCAG52150.043.8751756
CGCAGAG57850.037.9249768
TATCAAC61800.037.141282
ACGCAGA60100.037.1075557
GTACATG59900.031.5688821
ACATGGG54400.029.5488223
GCAGAGT72800.029.2416619
TACATGG61750.028.494132
GAGTACA42200.022.6624571
ATACCGT5500.022.380636
CATGGGG33700.021.7008914
TATACAG28350.020.1771645
TACCGTC2205.957694E-419.7476147
ATGGGCG3701.5934711E-719.5697085
CAGAGTA73950.019.4850259
Produced by FastQC (version 0.11.5)