FastQCFastQC Report
Thu 2 Jun 2016
DRR028503_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028503_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096196
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32270.1539455279945196No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC27700.059.5926934
ATCAACG28900.056.847913
CAACGCA29150.056.3769655
AACGCAG32400.050.483276
GTATCAA34700.050.1060331
GGTATCA19050.047.9550321
GTACATG60000.044.4492871
ACGCAGA35450.043.6458057
CGCAGAG34800.042.9532668
TATCAAC41600.040.5544972
TACATGG62250.040.2382
ACATGGG63250.038.438013
CATGGGA35950.031.157994
CGATACC13550.030.777033145
GCAGAGT45950.029.5307759
CATGGGG33300.028.7689384
GAGTACA42250.024.4123081
TAGACCG1650.002366209422.3316845
GTATATA21800.022.3046251
TATACCG2154.5307315E-420.5659225
Produced by FastQC (version 0.11.5)