FastQCFastQC Report
Thu 2 Jun 2016
DRR028503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096196
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24850.074.363961
GTATCAA47750.053.8770331
TCAACGC48650.050.754934
CAACGCA50100.049.2859765
ATCAACG50200.049.1877983
AACGCAG54750.045.364566
TATCAAC58250.043.136042
GTACATG60700.040.3530851
ACGCAGA60350.040.315197
CGCAGAG60300.039.628118
TACATGG62350.038.557452
ACATGGG61600.038.0864943
CATGGGA36500.031.7419664
GCAGAGT73550.031.30779
CTGCGCG2051.3034414E-524.725839
CATGGGG33750.024.6735024
TAGTACG2401.6165977E-624.1371214
GAGTACA42400.022.3900031
CAGAGTA73300.021.0417759
ACCGTAT4155.3171425E-717.4485238
Produced by FastQC (version 0.11.5)