FastQCFastQC Report
Thu 2 Jun 2016
DRR028502_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028502_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584526
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8770.15003609762439993No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6320.10812179441119812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6300.0109.94631
GTATCAA17050.059.6414261
ATCAGCG552.968502E-453.5955664
TCAACGC17450.053.211644
CAACGCA17450.052.7986225
ATCAACG17650.052.5994153
TACATGG35850.052.2035032
GTACATG37150.052.166321
AACGCAG19000.047.70296
TGGTATC2050.046.7244872
GCTCTAG1306.359187E-945.34611
CATGGGA20450.043.9640924
ACGCAGA20850.042.0566067
CGCAGAG20450.041.899268
ACATGGG40750.041.4059683
ATAGATG3750.041.264956
GTGGTAT2350.040.763251
GCGCAGA750.001471504138.7272268
TATCAAC26250.038.1737372
GATGACC3050.038.0923549
Produced by FastQC (version 0.11.5)