FastQCFastQC Report
Thu 2 Jun 2016
DRR028502_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028502_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584526
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29070.4973260385337863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27110.46379459596322486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15000.25661818293797023No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG9800.16765721285280724No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG7140.12215025507847382No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC6150.1052134550045678No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT6030.10316050954106404No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15150.0116.2256241
AGCAACG256.498951E-6115.8692254
ACGCACA300.001944018372.418277
GCAACGC406.6982015E-572.418275
GTGGTAT3100.070.124181
GTATCAA36100.067.242811
TGGTATC3650.057.5377962
ATCAACG46100.051.6824463
TCAACGC46550.051.1828354
CAACGCA46750.051.118785
GCTCTAG1351.9099389E-1048.3077661
AACGCAG49700.047.6474276
GATGACC3200.047.5244879
TACATGG34000.047.0718732
GTACATG36100.045.3638081
TATCAAC53850.044.9168052
ACGCAGA53450.043.762587
CGCAGAG53650.043.734428
ATAGATG3500.043.450966
CATGGGT2800.041.3818634
Produced by FastQC (version 0.11.5)