FastQCFastQC Report
Thu 2 Jun 2016
DRR028501_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028501_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332610
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20860.15653492019420534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11300.061.360471
TCAACGC18700.055.2365384
ATCAACG18700.055.2258573
CAACGCA19750.052.301935
GTATCAA21550.051.343241
AACGCAG21200.048.717146
GTACATG39400.041.936391
TACATGG41600.038.301562
ACGCAGA25600.038.0385177
TATCAAC27350.037.7595442
CGCAGAG25200.036.888048
ACATGGG43150.034.703343
GCAGAGT30000.028.5662639
CATGGGG28750.027.972334
CATGGGA22950.025.7185974
GAGTACA29900.023.929841
AGTACAT32500.020.4274982
TAGATCG2307.0773106E-419.2477595
CGATACC10300.018.839289145
ATAATAC12150.018.8209483
Produced by FastQC (version 0.11.5)