FastQCFastQC Report
Thu 2 Jun 2016
DRR028501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332610
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14230.10678292973938361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17000.065.63081
GTATCAA29750.053.332631
TCAACGC30450.050.886384
ATCAACG31600.049.4927673
CAACGCA32850.047.3890655
AACGCAG34700.045.0712246
TATCAAC38350.041.3479352
GTACATG42100.038.2038151
ACGCAGA41650.037.202657
CGCAGAG42550.036.0754178
TACATGG44550.035.4310382
TAGTACG2455.638867E-1132.5088544
ACATGGG46500.032.2323883
GCAGAGT50050.028.7888329
CATGGGA24150.027.2834554
GAGTACA31350.024.7276061
CATGGGG28650.022.9980954
AGTACAT34000.022.3607032
CTATACC7150.022.2787974
CAGAGTA50650.021.5860189
Produced by FastQC (version 0.11.5)