FastQCFastQC Report
Thu 2 Jun 2016
DRR028500_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028500_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580864
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24640.15586413505526092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.057.736391
TCAACGC20450.051.908744
ATCAACG21000.050.8820423
CAACGCA22400.046.0750165
GTATCAA24900.044.691171
AACGCAG23600.043.7222566
GTACATG40300.038.4023971
CGCAGAG25550.037.5168
ACGCAGA26550.036.6446047
TATCAAC29850.036.291342
TACATGG41850.036.0984342
ACATGGG47300.029.2895413
GCAGAGT34000.024.1365289
CGATACC10400.023.501797145
CATGGGG30900.022.1868574
CATGGGA22600.022.1805234
GAGTACA29650.021.6241321
GTATTGG10950.018.8446481
AGTACAT33400.018.533732
GTATTAG12650.018.0598981
Produced by FastQC (version 0.11.5)