FastQCFastQC Report
Thu 2 Jun 2016
DRR028500_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028500_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580864
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21620.1367606574632606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18280.11563297032508804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.072.300511
GTATCAA39250.061.2957271
ATCAACG44500.053.3705943
TCAACGC44650.053.0291334
CAACGCA46000.051.4728435
AACGCAG49450.048.3209956
TATCAAC52700.044.9288562
ACGCAGA56300.042.055967
CGCAGAG56600.041.449268
GTACATG43400.033.5612451
GCAGAGT67150.032.7805149
TACATGG44950.031.2507362
TATGCGA950.00475748330.4876985
ACATGGG45850.028.4263693
CAGAGTA63450.023.5084389
CATGGGA20800.020.5388854
GTGGTAT11200.019.4104211
CATGGGG32100.018.7223874
GTATTAT14050.018.5676921
TGAACCG2359.281897E-418.4872195
Produced by FastQC (version 0.11.5)