FastQCFastQC Report
Thu 2 Jun 2016
DRR028499_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028499_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1677617
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26120.15569703931231027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30750.055.8108671
TCAACGC29950.055.1051644
GGTATCA17600.054.4049761
ATCAACG30950.053.073633
CAACGCA31150.052.272755
AACGCAG33600.048.2330366
TATCAAC36300.045.6559262
ACGCAGA37600.041.53587
CGCAGAG37050.040.151158
GTACATG53650.037.2055361
TACATGG56300.032.4462552
ACATGGG61200.028.7661043
GCAGAGT44850.028.4770959
CATGGGA28950.025.450284
CGATACC11100.023.31795145
GTATAGA10300.020.7380891
GTATTAG12250.020.4432891
CATGGGG40500.020.3753054
GAGTACA41300.020.1528431
GTATATA16550.018.6921481
Produced by FastQC (version 0.11.5)