FastQCFastQC Report
Thu 2 Jun 2016
DRR028499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1677617
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33740.20111861050525837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28590.17042030451527376No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25600.076.4319151
GTATCAA47150.061.786741
ATCAACG55650.051.9231263
TCAACGC56050.051.4233744
CAACGCA57800.049.991735
AACGCAG59950.047.8364646
TATCAAC61200.047.2144132
ACGCAGA70800.040.0964477
CGCAGAG70900.039.427048
GTACATG52600.034.5811041
GCAGAGT82350.032.0103849
TACATGG58450.031.3465042
ACATGGG60750.029.086853
CATGGGA29200.025.049074
GTGGTAT12000.024.1562581
CAGAGTA77050.023.7794049
GAGTACA41250.022.487281
CATGGGG39700.022.0723194
GTATTAA18800.019.6590841
AGTACAT45300.019.0242
Produced by FastQC (version 0.11.5)