FastQCFastQC Report
Thu 2 Jun 2016
DRR028498_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028498_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535901
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22850.14877260969294245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.060.412351
GTATCAA27900.054.405231
ATCAACG26650.054.1830863
TCAACGC26950.053.3333664
CAACGCA28300.050.2682765
AACGCAG30150.046.683926
TATCAAC34550.043.073312
GTACATG50500.041.0008241
TACATGG51800.039.254032
ACGCAGA34450.039.148247
CGCAGAG33350.038.313768
ACATGGG55650.035.611643
GCAGAGT37400.030.6727079
CGATACC9400.030.604855145
CATGGGA28600.029.1229634
CATGGGG33050.027.4319554
GAGTACA36000.023.7428651
CGACGAA3203.6887286E-822.47544145
GTATAAA17200.020.1347891
AGTACAT39900.019.7567632
Produced by FastQC (version 0.11.5)