FastQCFastQC Report
Thu 2 Jun 2016
DRR028498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535901
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28100.18295450032261193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23810.15502301255093917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23100.084.374321
GTATCAA46100.059.881691
TCAACGC52800.052.110194
ATCAACG53450.051.7474143
CAACGCA54200.050.7641685
AACGCAG57350.047.72346
TATCAAC61600.044.548342
ACGCAGA65900.040.652697
CGCAGAG66800.039.6714068
GTACATG50450.038.633241
TACATGG52900.035.997562
GCAGAGT75750.034.219489
ACATGGG54500.033.0807883
CATGGGA28550.028.6579634
CATGGGG31300.025.4461064
CAGAGTA73350.025.2704439
GTGGTAT10600.023.923911
GAGTACA38850.021.0745071
AGTACAT41150.019.1791632
TACCCCG2851.6500779E-417.7838675
Produced by FastQC (version 0.11.5)