FastQCFastQC Report
Thu 2 Jun 2016
DRR028497_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028497_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538393
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24600.15990712386236805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16200.051.4321984
ATCAACG17000.049.0036623
CAACGCA17150.048.1548585
AACGCAG18150.044.68026
GTATCAA21150.042.1726041
GGTATCA13700.041.9690861
ACGCAGA21000.035.8079877
CGCAGAG21400.035.2776958
CGATACC10500.034.922394145
GTACATG32100.034.905511
TATCAAC25300.033.2165342
TACATGG34950.031.6383652
ACATGGG36150.028.95873
GCAGAGT29800.022.6548739
CATGGGG26900.021.6544174
CGACGAA3055.7893703E-721.216114145
GTATAAG9600.020.7323231
GTATTAG12300.018.578561
ACCGTGC4003.5201083E-718.1476578
GTATAGG7350.018.0526351
Produced by FastQC (version 0.11.5)