FastQCFastQC Report
Thu 2 Jun 2016
DRR028497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538393
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19450.12643063248467717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17840.11596516624815635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20800.070.375421
GTATCAA37400.060.452741
TCAACGC40550.054.818544
ATCAACG41100.054.261133
CAACGCA41400.053.867935
AACGCAG43750.050.8089566
TATCAAC47850.047.2120252
ACGCAGA49050.044.58087
CGCAGAG49650.043.6045538
GCAGAGT59000.035.0989389
GTACATG31650.030.9095761
TAGCGCA1306.5334514E-427.8488089
TACATGG35100.027.4362342
GTGGTAT12700.025.6768511
TGGTATC11600.025.5920932
ACATGGG35250.024.6491583
GCACCGT2401.6169979E-624.1356326
TAGATCG1700.003116683621.2961465
GTATAGA10550.021.293261
CATGGGG24450.020.7299924
Produced by FastQC (version 0.11.5)