FastQCFastQC Report
Thu 2 Jun 2016
DRR028496_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028496_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232704
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18860.15299698873371062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17650.051.6495364
CAACGCA18200.050.4947365
ATCAACG19000.048.354583
GGTATCA12650.048.220611
GTATCAA20700.047.2200471
AACGCAG19450.045.729996
GTACATG33600.042.6521031
TATCAAC24250.040.916952
TACATGG35800.037.7762
ACGCAGA22500.036.9189457
ACATGGG38850.035.377883
TAGACCG850.00256058834.5978055
CGCAGAG23350.034.1524288
GAGTACA25950.028.0377391
CGATACC7250.027.827566145
CATGGGA18700.026.7335574
CATGGGG25800.026.2154664
AGTACAT26300.024.3133452
GCAGAGT27650.024.1227199
GTATTAG8600.020.5096341
Produced by FastQC (version 0.11.5)